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The significance of indole is further underscored by the fact that it is also the side-chain of L-tryptophan, one of only 21 proteinogenic amino acids found in eukaryotes

doi: 10.1016/0006-2952(93)90437-2

Cells were pelleted at 300 g for 6 min, resuspended in complete MEM with 50 g ml 1 Gen at 2.5 10 5 cells per ml, and dispensed (80 l per well) into 384-well white and black plates (ThermoFisher) for luminescence and fluorescence measurements, respectively